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Related Experiment Videos

Elevated basic fibroblast growth factor levels in stroke-prone spontaneously hypertensive rats

T N Lin1, Y P Wong, J J Chen

  • 1Institute of Biomedical Sciences, Academia Sinica, Taipei, ROC.

Neuroscience
|January 1, 1997
PubMed
Summary

Basic fibroblast growth factor (bFGF) levels increase with age in stroke-prone rats, correlating with stroke incidence. This suggests bFGF may play a role in stroke development and cerebral lesions.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Basic fibroblast growth factor (bFGF) is a polypeptide with known mitogenic, angiogenic, and neurotrophic properties.
  • Stroke-prone spontaneously hypertensive rats (SHR-SP) are a model for studying stroke pathogenesis.

Purpose of the Study:

  • To investigate the temporal and spatial expression of bFGF in SHR-SP rats compared to spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats.
  • To determine the correlation between bFGF expression and stroke incidence in SHR-SP rats.

Main Methods:

  • Northern blot analysis to quantify bFGF messenger RNA (mRNA) levels.
  • Immunohistochemical studies to assess bFGF protein expression and localization.
  • Comparison of bFGF expression across different ages and rat strains (SHR-SP, SHR, WKY).

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Main Results:

  • Higher total RNA concentration in the cerebral cortex of young SHR-SP rats compared to SHR and WKY rats.
  • Age-dependent increase in bFGF mRNA in the cerebral cortex of SHR-SP rats, but not in SHR or WKY rats.
  • Significant increase in bFGF protein immunoreactivity in the cerebral cortex of aged SHR-SP rats, primarily in astrocytes, which decreased with severe tissue damage.

Conclusions:

  • Aged SHR-SP rats exhibit significantly increased bFGF mRNA and protein expression, correlating with a high incidence of stroke.
  • bFGF may play a role in the development of cerebral lesions in SHR-SP rats.
  • The observed increase in bFGF is specific to aged stroke-prone rats and its expression changes with tissue damage severity.