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Updated: Aug 15, 2026

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
Published on: May 20, 2015
Cell-cycle regulated expression of Rap1 in regenerating liver
J L Cruise1, M P Rafferty, M M Riehle
1Department of Biology, University of St. Thomas, St. Paul, Minnesota 55105, USA. jlcruise@stthomas.edu
Abstract:
Rap1 proteins are capable of competing with Ras p21 for binding to effectors, and of antagonizing some Ras-induced effects, but their participation in normal growth regulation has not been established. The level of Rap1 protein and the expression of the rap1A gene were examined by immunoblotting and Northern analysis during the regenerative growth response in rat liver following partial hepatectomy. Protein and mRNA were significantly down-regulated prior to and during the onset of DNA synthesis. The timing of this effect is consistent with a model in which expression of Rap1 is turned off or down to allow the initiation of proliferation.
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