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Free cytosolic calcium levels modify intracellular pH in rat pancreatic acini
A González1, P J Camello, J A Pariente
1Department of Physiology, University of Extremadura, Caceres, Spain.
Abstract:
We have used BCECF- or Fura-2-loaded rat pancreatic acinar cells to investigate the relationship between Ca2+ mobilization and intracellular pH (pHi). Ca2+-mobilizing agonists CCK-8 and ACh induced a transient acidification totally dependent on release of Ca2+ from internal stores. Employment of different physiological tools including ionomycin and thapsigargin to increase the cytosolic Ca2+ concentration and capacitative calcium influx also induced cellular acidification. Application of 1mM LaCl3 reduced the CCK-8-evoked acidification. These data indicate that the mobilization of intracellular Ca2+ stores by CCK-8 decreases cellular pH by Ca2+/H+ exchanger.