Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quadruple immunofluorescence: a direct visualization method

G L Ferri1, R M Gaudio, I F Castello

  • 1Department of Cytomorphology, University of Cagliari, Italy.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|February 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: A novel oncogenic BTK isoform is overexpressed in colon cancers and required for RAS-mediated transformation.

Oncogene·2024
Same author

Search for long-lived particles decaying to leptons with large impact parameter in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022
Same author

[CD8<sup>+</sup> T cells and fibrocytes: dangerous interplay in the distal bronchi of patients with chronic obstructive pulmonary disease?]

Revue des maladies respiratoires·2022
Same author

Evidence for X(3872) in Pb-Pb Collisions and Studies of its Prompt Production at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2022
Same author

Search for Long-Lived Particles Decaying in the CMS End Cap Muon Detectors in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2022
Same author

Measurements of the Electroweak Diboson Production Cross Sections in Proton-Proton Collisions at sqrt[s]=5.02  TeV Using Leptonic Decays.

Physical review letters·2021

This study presents a novel fluorescence immunostaining technique for simultaneously visualizing four antigens in a single tissue section. Optimized filters enable clear, distinct imaging of each fluorochrome without signal interference, advancing multiplexed biomarker detection.

Area of Science:

  • Biochemistry
  • Immunology
  • Microscopy

Background:

  • Multiplexed fluorescence imaging is crucial for analyzing complex biological systems.
  • Existing methods often suffer from spectral overlap and signal crosstalk.
  • There is a need for robust techniques to detect multiple targets in a single sample.

Purpose of the Study:

  • To develop and validate a novel fluorescence immunostaining protocol.
  • To enable the simultaneous detection of four distinct antigens within the same tissue section.
  • To demonstrate selective visualization of multiple fluorochromes with minimal spectral interference.

Main Methods:

  • Utilized fluorescence immunostaining targeting four different antigens.
  • Employed fluorochrome conjugates with distinct emission spectra (blue, green, yellow, red).

Related Experiment Videos

  • Developed and applied specialized single fluorochrome filter combinations for sequential imaging.

    • Main Results:

    • Successfully visualized four antigens in a single tissue section.
    • Achieved selective excitation and emission detection for each fluorochrome.
    • Demonstrated the absence of visible crosstalk between the different fluorescent labels.
    • Confirmed the utility of designed filter combinations for multiplexed imaging.

    Conclusions:

    • The described fluorescence immunostaining method allows for precise multiplexed detection of multiple antigens.
    • The specialized filter sets effectively prevent spectral overlap, ensuring high-quality imaging.
    • This technique offers a valuable tool for advanced biomarker research and diagnostics.