Studies on the stability of HSV-1 temperature sensitive 28 degrees C clone

The temperature mutants of herpes simplex virus type 1 has been received. The following properties on different levels of passages were investigated: stability of virulence, virus infection titer and immunogenicity. The virulence stability was expressed by percent of survivors and mean survival time in infected mice (CFW/Pzh and BALB/cPzh strains). Virus infection titer's stability was measured after prolonged exposure of temperature sensitive mutant (ts 28 degrees C) to critical temperatures (+4 degrees C and from +20 degrees C to +23 degrees C). Stability of immunogenic potency of ts 28 degrees C was evaluated in mice immunized with the virus on different level of passages and after that they were challenged with highly pathogenic temperature resistant clone (tr 39 degrees C). The performed experiments proved the stability of investigated features.