Voltage-dependent potassium currents of sympathetic preganglionic neurons in neonatal rat spinal cord thin slices

Voltage-dependent potassium currents were analyzed in the visually identified sympathetic preganglionic neurons (SPNs) of neonatal rat spinal cord thin slices by the whole-cell patch-clamp technique. Some of the SPNs were identified by the presence of retrogradely transported fluorescent dye, DiI, injected into the superior cervical ganglion several days prior to experimentation. In a tetrodotoxin (TTX)-containing solution, a step depolarization from the holding potential of -72 mV generated a slow outward current that was suppressed by tetraethylammonium (TEA) and by Ca(2+)-free/2.5 ImM Co2+ solution. Ca(2+)-dependent current consisted of a transient and a sustained components. In a Ca(2+)-free (substituted with Mg2+) solution with TTX and TEA, a step depolarization from a hyperpolarized potential evoked a transient outward current that was blocked by 4-aminopyridine (4-AP). A step hyperpolarization evoked a voltage-dependent inward current, the conductance of which was dependent not only on the membrane potential, but also on the extracellular K+ concentration. Tail current analyses revealed that all of these currents were carried by K+ ions. These results indicate that SPN possesses at least five types of voltage-dependent K+ current, including the delayed rectifier current (IK), Ca(2+)-dependent transient current (IC), Ca(2+)-dependent sustained current (IAHP), A-current (IA) and inward rectifying current (Iu), which may be targets of putative transmitters released from various descending and segmental inputs impinging upon the SPN.