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Identification of a long huntingtin mRNA transcript in mouse brain
E Casanova1, A Alonso-Llamazares, D Zamanillo
1Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
Abstract:
Two mRNA species of the Huntington disease (HD) gene that share identical protein coding sequences but differ in their 3' untranslated region have been identified in human. Although a similar situation has been suggested to occur in mouse, only one cDNA has been isolated to date. We report the isolation of a novel partial cDNA of the mouse HD gene that is identical in its protein coding sequence to the previously reported cDNA, although it differs in the distal portion of the 3' untranslated region. Northern blotting assays indicate that this mRNA transcript is preferentially expressed in brain, with highest levels in cerebellum, cerebral cortex and striatum.