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delta IK17: an antigen expressed on human lymphocytes
R Sotiriadou1, D Kokkinopoulos, J Stinios
1Department of Immunology Regional Anticancer, Oncologic Hospital of Athens, Saint Savas, Greece.
The International Journal of Biological Markers
|October 1, 1996
Summary
A novel monoclonal antibody, delta IK17, targets a cell surface protein on human lymphocytes. This antibody aids in understanding lymphocyte maturation and function, with potential implications for hematological diseases.
Area of Science:
- Immunology
- Cell Biology
Background:
- The delta IK17 monoclonal antibody was developed to identify and characterize specific cell surface proteins on human lymphocytes.
- Understanding lymphocyte surface markers is crucial for dissecting immune cell development and function.
Purpose of the Study:
- To produce and characterize the anti-delta IK17 monoclonal antibody.
- To investigate the expression patterns of the delta IK17 antigen on human lymphocytes throughout maturation and in different immune cell subsets.
- To explore the functional role of delta IK17 expression in T cell responses and its relevance in hematological diseases.
Main Methods:
- Production of monoclonal antibody via myeloma fusion with immunized mouse spleen cells.
- Detection of the 44kD cell surface protein using the delta IK17 antibody on human lymphocytes.
- Analysis of antigen expression across lymphocyte maturation stages, T cell subsets (CD4+, CD8+), B cells, NK cells, and activated cells.
- Assessment of T cell function through TPA stimulation and PWM-induced IgM synthesis assays.
- Evaluation of delta IK17 expression levels in various hematological diseases.
Main Results:
- The delta IK17 antibody recognizes a 44kD cell surface protein expressed on human thymocytes, CD4+, CD8+ T cells, B cells, NK cells, and activated cells.
- Antigen expression is observed across early, intermediate, and late stages of lymphocyte maturation and correlates with ontogenesis.
- A delta IK17+ T cell subpopulation exhibited diminished response to TPA stimulation but enhanced helper function for IgM synthesis compared to delta IK17- cells.
- Altered levels of delta IK17 expression were found in several tested hematological diseases.
Conclusions:
- The delta IK17 antibody identifies a significant cell surface marker involved in human lymphocyte development and function.
- The antigen's expression pattern suggests a role in lymphocyte maturation and immune regulation.
- Delta IK17 expression may serve as a potential biomarker for certain hematological conditions.