Special transformation processes using fungal spores and immobilized cells

Although many microbial processes have been described which are able to produce interesting aroma compounds, the number of industrial applications are limited. Reasons for this are in most cases low final product yield, low biotransformation rates, substrates and/or end-products inhibition, toxicity towards the microorganisms themselves and difficulties of recovery from the bioreaction mixture. This means that the development of specific catalysts and processes is an important challenge for researchers in this field. This review presents two special kinds of catalysts, fungal spores and immobilized cells, with emphasis on their production and on their use in the production of aroma compounds. The production of fungal spores by solid state fermentation is described in greater detail. In the second part, this review also offers examples of development of three production processes, the production of methyl ketones of spores of Penicillium roquefortii, the hydroxylation of beta-ionone by immobilized Aspergillus niger cells, and the production of alkyl pyrazines by bacteria in liquid and solid media. For each of these processes, the analysis of limiting steps-biological and/or physico-chemical-is presented and the significant role of process conditions to increase aroma yield is discussed.