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Simple hemorrhage induces tissue factor mRNA in the liver
M Yamashita1, J Tanaka, M Yamashita
1Department of Emergency Medicine, University of Tsukuba School of Medicine, Japan.
Surgery Today
|January 1, 1997
Summary
Large hemorrhage can induce hypercoagulability, shortening blood clotting time. This study found increased tissue factor (TF) mRNA in the liver following hemorrhage, suggesting TF induction contributes to clotting responses.
Area of Science:
- Physiology
- Hematology
- Molecular Biology
Background:
- Thrombotic events can occur after significant blood loss (hemorrhage).
- The exact mechanisms linking hemorrhage to thrombosis remain unclear.
- Previous research indicated elevated plasminogen activator inhibitor-1 after hemorrhage.
Purpose of the Study:
- To investigate the coagulation system's response to hemorrhage.
- To measure changes in whole blood clotting time.
- To assess the expression of messenger RNA (mRNA) for tissue factor (TF) in the liver post-hemorrhage.
Main Methods:
- Rats underwent hemorrhage (20 ml/kg blood withdrawal).
- Whole blood clotting time was determined using the Lee-White method.
- Liver TF mRNA levels were quantified via reverse transcription polymerase chain reaction (RT-PCR) and high-performance liquid chromatography (HPLC).
Main Results:
- Hemorrhage significantly shortened whole blood clotting time.
- A significant increase in liver TF mRNA was observed at 1, 2, and 4 hours post-hemorrhage compared to controls.
- These changes occurred even without associated tissue trauma.
Conclusions:
- Simple hemorrhage, without tissue injury, can induce a hypercoagulable state.
- The induction of tissue factor (TF) appears to play a role in the hypercoagulability observed after hemorrhage.
- These findings provide insights into the pro-thrombotic mechanisms following significant blood loss.