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Cytokine mRNA expression after transient and prolonged hypotension
M Yamashita1, J Tanaka, M Yamashita
1Department of Emergency Medicine, University of Tsukuba School of Medicine, Amakubo, Japan.
Surgery Today
|January 1, 1997
Summary
Hemorrhagic shock impacts cytokine messenger RNA (mRNA) levels in the liver. Prolonged hypotension persistently elevates tumor necrosis factor alpha and interleukin-1beta mRNA, unlike transient hypotension.
Area of Science:
- Biochemistry
- Immunology
- Physiology
Background:
- The role of cytokines in hemorrhagic shock is debated, with conflicting research on their elevation.
- Understanding cytokine responses is crucial for managing shock and its complications.
Purpose of the Study:
- To investigate changes in tumor necrosis factor (TNF) activity and mRNA levels of TNF alpha, interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) following transient and prolonged hypotension.
- To differentiate cytokine responses based on the duration of hypotension.
Main Methods:
- Induction of transient hypotension (TH) and prolonged hypotension (PH) in rats.
- Measurement of serum TNF activity using cytotoxicity assays.
- Quantification of liver cytokine mRNA (TNF alpha, IL-1beta, IL-6) via RT-PCR and HPLC.
Main Results:
- Serum TNF activity did not significantly increase in either TH or PH groups.
- TNF alpha and IL-1beta mRNA showed transient elevation in the TH group and persistent elevation in the PH group.
- IL-6 mRNA increased significantly only in the PH group.
Conclusions:
- Hemorrhage induces cytokine mRNA expression in the liver.
- The duration of hypotension significantly influences the persistence of cytokine mRNA elevation, with prolonged hypotension leading to sustained increases in TNF alpha, IL-1beta, and IL-6 mRNA.