Serological analysis of monoclonal anti-E and -e with Japanese E/e variant red cells

The agglutination patterns have been analyzed for the reaction between 24 monoclonal antibodies (MAB) with specificity for the Rh antigen E or e and red cells of E/e variant from Japanese blood donors. The MABs were tested for direct agglutination of papain treated cells at 20 degrees C. The reactions of a full titration series of each MAB with a E/e variant cell were compared to the reactions of that MAB with normal E + e + cells. Sixteen anti-E were divided into a minimum of 6 different agglutination patterns with 8 examples of E variant cells. Eight anti-e gave a minimum of 5 different agglutination patterns with 6 examples of e variant cells.