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IgG anti-D MAbs in tests by flow cytometry

F Roubinet1, A Blancher

  • 1Laboratoire d'Immunohématologie, Etablissement de Transfusion Sanguine Pyrénées Garonne, Toulouse, France.

Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine
|January 1, 1996
PubMed
Summary

This study compared 72 IgG anti-D monoclonal antibodies (Mabs) using flow cytometry and indirect immunofluorescence against various D variant red blood cells (RBCs). Results were validated against the indirect antiglobulin test (IAT).

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Area of Science:

  • Immunology
  • Hematology
  • Blood group serology

Background:

  • Monoclonal antibodies (Mabs) targeting the D antigen are crucial for blood transfusion compatibility.
  • Understanding the reactivity of anti-D Mabs with D variant red blood cells (RBCs) is essential for accurate blood typing.

Purpose of the Study:

  • To evaluate the performance of 72 IgG anti-D Mabs against a panel of D variant RBCs.
  • To compare flow cytometry (FCM) and indirect immunofluorescence methods with the indirect antiglobulin test (IAT) for anti-D Mab characterization.

Main Methods:

  • Seventy-two IgG anti-D Mabs were tested.
  • Indirect immunofluorescence and flow cytometry (FCM) were employed.
  • Reactions were assessed using R1r, rr, and D variant RBCs (DIIIb, DVI, DVII, Rh33, and ISBT 48).

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Main Results:

  • The study characterized the reactivity profiles of numerous anti-D Mabs.
  • Flow cytometry and indirect immunofluorescence showed comparable results to the indirect antiglobulin test (IAT).
  • Specific D variant phenotypes were identified as targets for different Mabs.

Conclusions:

  • Flow cytometry and indirect immunofluorescence are reliable methods for characterizing anti-D Mabs.
  • Accurate identification of anti-D Mabs is critical for managing transfusion in patients with D variants.