Separation and partial purification of beta-glucosidase and two endoglucanases in Aspergillus niveus

The thermotolerant Aspergillus niveus strain RMF 7883 was grown in Czapek medium, with filter paper cellulose. The proportion of mycelial-bound to extracellular enzymes was studied. Most of the beta-glucosidase (80.9%) and endoglucanases (78.3%) activities were extracellular. The extracellular endoglucanases and beta-glucosidase were separated and partially purified by Sephadex G-100 gel filtration, followed by ion exchange chromatography on CM-trisacryl M. Two extracellular endoglucanases, EG I and EG II (130 kDa and 35 kDa, respectively), and beta-glucosidase (194 kDa) were isolated from culture filtrate.