Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mechanisms for solvent tolerance in bacteria

J L Ramos1, E Duque, J J Rodríguez-Herva

  • 1Department of Organic Chemistry, University of Granada, 18012 Granada, Spain.

The Journal of Biological Chemistry
|February 14, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In memoriam: Álex Urbistondo.

Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica·2025
Same author

Anthelmintic activity and chemical profile of native plant extracts from the Yucatan Peninsula against Toxocara canis.

Acta tropica·2024
Same author

A hierarchical dataset of vegetative and reproductive growth in apple tree organs under conventional and non-limited carbon resources.

Data in brief·2023
Same author

Assessing optimal frequency for image acquisition in computer vision systems developed to monitor feeding behavior of group-housed Holstein heifers.

Journal of dairy science·2022
Same author

Corrigendum to 'First-line nivolumab plus ipilimumab with 2 cycles of chemotherapy versus chemotherapy alone (4 cycles) in advanced non-small cell lung cancer: CheckMate 9LA 2-year update': [ESMO Open Volume 6, Issue 5, October 2021, 100273].

ESMO open·2021
Same author

First-line nivolumab plus ipilimumab with two cycles of chemotherapy versus chemotherapy alone (four cycles) in advanced non-small-cell lung cancer: CheckMate 9LA 2-year update.

ESMO open·2021
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
See all related articles

Pseudomonas putida DOT-T1 develops tolerance to toxic compounds through membrane rigidity changes. These involve fatty acid transformations and altered phospholipid levels, preventing solvent penetration.

Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Pseudomonas putida DOT-T1 exhibits tolerance to toxic solvents like toluene.
  • Understanding the mechanisms of this tolerance is crucial for bioremediation and industrial applications.

Purpose of the Study:

  • To elucidate the short- and long-term molecular mechanisms underlying Pseudomonas putida DOT-T1's tolerance to toluene.
  • To identify key cellular components and processes involved in solvent resistance.

Main Methods:

  • Analysis of changes in cell membrane fatty acid composition (cis- and trans- isomers).
  • Quantification of phospholipid head group alterations (cardiolipin, phosphatidylethanolamine).
  • Investigation of toluene exclusion and metabolic degradation pathways.

Related Experiment Videos

  • Characterization of mutant strains with impaired tolerance mechanisms.

    • Main Results:

    • Short-term tolerance involves rapid cis-fatty acid isomerization increasing membrane rigidity.
    • Long-term tolerance includes changes in cardiolipin and phosphatidylethanolamine levels, further enhancing membrane rigidity.
    • Biochemical mechanisms like solvent exclusion systems and metabolic oxidation reduce intracellular toluene concentrations.
    • Mutants lacking cis-trans isomerization, OprL protein, or toluene exclusion are hypersensitive to toluene.

    Conclusions:

    • Cell membrane physical alterations (rigidity) and biochemical mechanisms (exclusion, metabolism) are key to Pseudomonas putida DOT-T1's toluene tolerance.
    • These combined strategies effectively prevent solvent penetrance and toxicity.
    • Genetic and biochemical pathways identified are critical for survival in toluene-contaminated environments.