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A rapid method for measuring pericentric inversions using fluorescence in situ hybridization (FISH)
J N Lucas1, F S Hill, A M Chen
1Lawrence Livermore National Laboratory, University of California, Livermore 94551, USA.
International Journal of Radiation Biology
|January 1, 1997
Summary
This study measured pericentric inversions in human cells exposed to gamma radiation. Results indicated that pericentric inversions occurred at the same frequency as centric rings.
Area of Science:
- Cytogenetics
- Radiation Biology
- Genomics
Background:
- Chromosomal aberrations, such as pericentric inversions, are critical indicators of DNA damage.
- Fluorescent in situ hybridization (FISH) is a powerful tool for detecting chromosomal rearrangements.
Purpose of the Study:
- To quantify pericentric inversion frequencies in human lymphocytes following gamma irradiation.
- To compare the frequency of pericentric inversions with other chromosomal aberrations like centric rings and translocations.
Main Methods:
- Human lymphocytes were exposed to 2.9 Gy of 60Co gamma radiation.
- Three-color FISH using probes for telomeric, subcentromeric, and centromeric regions was employed.
- Pericentric inversions were identified by the positional change of fluorescent signals relative to the centromere.
Main Results:
- The frequency of pericentric inversions was found to be equal to that of centric rings.
- The whole-genome F-ratio of apparently simple translocations to pericentric inversions was calculated to be 5.6.
Conclusions:
- Gamma irradiation induces pericentric inversions and centric rings at comparable frequencies in human lymphocytes.
- The study provides quantitative data on chromosomal aberration frequencies, contributing to radiation biodosimetry and understanding genomic instability.