Related Experiment Videos
Photoaffinity labelling of a DNA-binding site on the globular domain of histone H5
F A Goytisolo1, L C Packman, J O Thomas
1Cambridge Centre for Molecular Recognition, University of Cambridge, UK.
European Journal of Biochemistry
|December 15, 1996
Abstract:
We have labelled a DNA-binding site on the globular domain of histone H5 (GH5) by ultraviolet-activated cross-linking of a self-complementary 5-bromodeoxyuridine (5BrU)-substituted oligonucleotide with the sequence 5'-AGCGA5BrUATCGCT-3'. Cross-linking was to His62, mainly to the protein backbone. This observation provides further support for the mode of binding of GH5 to DNA proposed on the basis of the similarity between the X-ray crystal structure of GH5 and the DNA-bound structures of catabolite activator protein and hepatic nuclear factor 3 gamma [Ramakrishnan, V. (1994) Curr. Opin. Struct. Biol. 4. 44-50].