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Laser scanning cytometry in pathology of solid tumors. A review
W Gorczyca1, Z Darzynkiewicz, M R Melamed
1Department of Pathology, New York Medical College, Valhalla 10595, USA.
Acta Cytologica
|January 1, 1997
Abstract
Objective:
To review application of laser scanning cytometry (LSC) to analyze several different parameters of human solid tumors in relation to the cell cycle.
Study Design:
Tissue sections of cytology specimens were stained for specific parameters and analyzed by LSC.
Results:
Examples of LSC analysis of the expression of ER, Ki-67, cyclin B1, BrdUrd and ploidy are given.
Conclusion:
LSC provides rapid, high-precision measurement of the chosen parameters or constituents of each cell in a population of cells and correlates those measurements with the visual image of the corresponding cell.