Structural polypeptides of different clinical strains of avian adenovirus type-1

The polypeptides of two liver strains, two ovarian strains and one standard strain of avian adenovirus-1 (AAV-1) were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot using polyclonal antibodies to all the strains and isoelectric focusing (IEF). All the strains had 11 polypeptides and molecular weight varied between 9 and 100 kDa. The polypeptide pattern was similar in all the strains as revealed by SDS-PAGE and Western blot. However, some differences among the strains on the basis of isoelectric point of polypeptide were observed by IEF.