Related Experiment Videos
Ly1- (CD5-) B cells produce interleukin (IL)-10
R S Gieni1, D T Umetsu, R H DeKruyff
1Department of Pediatrics, Stanford University, California 94305, USA.
Cellular Immunology
|February 1, 1997
Summary
Conventional B cells, specifically Ly-1- (CD5-) B cells, produce Interleukin-10 (IL-10) during immune responses. This IL-10 production by B cells may enhance their role in promoting humoral immunity.
Area of Science:
- Immunology
- Cell Biology
Background:
- Interleukin-10 (IL-10) is crucial for regulating humoral immunity.
- IL-10 inhibits macrophage activation and promotes Th2 cytokine production.
- The role of conventional B cells in IL-10 production was previously unclear.
Purpose of the Study:
- To investigate the capacity of conventional Ly-1- (CD5-) B cells to produce IL-10 protein.
- To determine if B cells contribute to IL-10 levels during immune activation.
Main Methods:
- Purification of splenic B cells from BALB/c and C57BL/6 mice.
- Stimulation of B cells with lipopolysaccharide (LPS) and antigen restimulation.
- Quantification of IL-10 production using ELISA and bioassays.
- Assessment of IL-10 production after removal of Ly-1+ B cells.
Main Results:
- Naive splenic B cells produced IL-10 when stimulated with LPS.
- Antigen-primed B cells also produced IL-10 upon in vitro restimulation.
- IL-10 production was primarily attributed to Ly-1- B cells, not Ly-1+ B cells.
Conclusions:
- Normal Ly-1- B cells are a significant source of IL-10 during immune responses.
- B cell-derived IL-10 may enhance the capacity of B cells to promote humoral immunity.