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TNF alpha concentrations and mRNA expression are increased in preeclamptic placentas
1Department of Obstetrics and Gynecology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0034, USA.
Journal of Reproductive Immunology
|December 1, 1996
Summary
Tumor necrosis factor-alpha (TNF alpha) is elevated in preeclamptic placentas, contributing to increased oxidative stress and lipid peroxidation. This finding highlights TNF alpha
Area of Science:
- Obstetrics and Gynecology
- Immunology
- Biochemistry
Background:
- Tumor necrosis factor-alpha (TNF alpha) is a cytokine involved in immunoregulation and cell modulation.
- Preeclamptic placentas exhibit increased lipid peroxides, indicating heightened oxidative stress.
- A potential link between elevated TNF alpha and oxidative stress in preeclampsia warrants investigation.
Purpose of the Study:
- To investigate whether TNF alpha protein and mRNA levels are increased in preeclamptic placentas compared to normal placentas.
- To determine the association between TNF alpha and lipid peroxidation in preeclamptic placentas.
Main Methods:
- Quantification of TNF alpha protein in placental tissue using ELISA.
- Measurement of TNF alpha and lipid peroxides in placental villi incubation medium.
- Assessment of TNF alpha mRNA expression via RT-PCR.
Main Results:
- Significantly higher TNF alpha tissue levels in preeclamptic placentas (P < 0.05).
- Elevated TNF alpha and lipid peroxide concentrations in incubation medium from preeclamptic placentas (P < 0.05).
- Positive correlation between TNF alpha and lipid peroxide concentrations in incubation medium (r = 0.608).
- TNF alpha mRNA detected in preeclamptic placentas but not in normal placentas.
Conclusions:
- Preeclamptic placentas show increased TNF alpha protein and mRNA expression.
- Elevated TNF alpha in preeclampsia is associated with heightened lipid peroxidation.