Glucosyltransferase activity of commercial juice-processing enzyme preparations

Of various commercial enzyme preparations examined, Cytolase M102 was found to contain the highest glucosyltransferase activity (55 U ml-1). It rapidly converted maltose to panose (Glc alpha-->6 Glc alpha l-->4 Glc) with a Vmax value of 5.8 mmol l-1 min-1 at 50 degrees C in 0.05 mol l-1 sodium acetate buffer (pH 4.4). The Km value of the enzyme for maltose was 750 mmol l-1. Yields of panose and glucose after 45 min of reaction, for example, were 47.2% and 52.8%, respectively, on the basis of the amount of maltose consumed.