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Related Experiment Videos

Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein

C Delenda1, S Hausmann, D Garcin

  • 1Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland.

Virology
|February 3, 1997
PubMed
Summary

Sendai virus V protein is not essential for virus replication. Removing the V protein and increasing the W protein resulted in a virus that replicated normally, suggesting V is an accessory protein.

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Area of Science:

  • Virology
  • Molecular Biology

Background:

  • The Sendai virus V protein, a nonstructural trans-frame protein, contains a conserved cysteine-rich zinc-binding domain fused to the N-terminal P protein via mRNA editing.
  • The P gene naturally expresses a W-like protein (N-terminal half of P) at low levels.

Purpose of the Study:

  • To investigate the role of the Sendai virus V protein in viral replication.
  • To determine if the V protein is essential for virus propagation.

Main Methods:

  • Engineered a Sendai virus with a translation stop codon at the V open reading frame (ORF) start.
  • Utilized a system for recovering infectious virus from cDNA.

Main Results:

  • The engineered V-minus virus, which produces a W-like protein, replicated normally in cell culture and embryonated chicken eggs.

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  • This indicates that the V protein is not essential for Sendai virus replication under these conditions.

    • Conclusions:

    • The Sendai virus V protein functions as an accessory protein.
    • The cysteine-rich Zn2+-binding domain likely plays a specialized role in virus propagation, but is not essential for replication itself.