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Related Experiment Videos

Direct purification of lysozyme using continuous counter-current expanded bed adsorption

R O Owen1, H A Chase

  • 1Department of Chemical Engineering, University of Cambridge, UK.

Journal of Chromatography. A
|January 3, 1997
PubMed
Summary

A novel continuous chromatography technique effectively purifies proteins from unclarified sources. This method, using a specialized adsorbent, yields highly purified and clarified protein streams, streamlining downstream processing.

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Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Separation Science

Background:

  • Protein purification is crucial for various applications.
  • Existing methods often require pre-clarification of feedstocks.
  • Continuous processing offers advantages in efficiency and scalability.

Purpose of the Study:

  • To introduce and evaluate a novel continuous counter-current chromatography technique for protein purification.
  • To assess the efficiency of the technique in handling unclarified feedstocks.
  • To demonstrate the purification of specific proteins, lysozyme, from complex biological samples.

Main Methods:

  • Application of continuous counter-current chromatography.
  • Utilized an adsorbent comprising a perfluorocarbon matrix coated with poly(vinyl alcohol) and derivatized with Procion Red HE-7B.

Related Experiment Videos

  • Purified lysozyme from egg-whites and enriched bovine milk.

    • Main Results:

    • Achieved continuous purification of lysozyme from egg-whites with 90.5% yield and a purification factor of 19.4.
    • Purified lysozyme from enriched bovine milk at a rate of 41000 U/min with 66.0% yield.
    • Generated fully clarified product streams from both feedstocks, suitable for direct downstream processing.

    Conclusions:

    • The novel continuous chromatography technique efficiently purifies proteins from unclarified sources.
    • The method provides high yields and purity, with clarified product streams.
    • This technique offers a promising approach for streamlined and scalable protein purification.