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Updated: Aug 25, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Quantitative evaluation of carbon isotopic fractionation during reversed-phase high-performance liquid chromatography
1Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853-1301, USA.
Abstract:
The fractionation of 13C during low-performance preparative LC and high-performance LC is reported quantitatively for methyl palmitate and using high-precision isotope ratio mass spectrometry (IR-MS). For both preparative and high-performance analytical columns, 13C enrichment is about 7% greater than the parent starting material, drops sharply in the first section of the peak and then settles to a value about 1% below that of the starting material. Recycling over a single HPLC column did not induce greater fractionation. These results emphasize the importance of quantitative peak collection for high-precision IR-MS studies, particularly the first part of the peak where the isotope ratio changes rapidly.
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