[The analysis of grandaksin in cadaveric material]

Grandaxine was measured in expert cadaveric material (gastric wall, small intestine, and kidney) by thin layer and liquid chromatography and UV spectroscopy. Chromatographic measurement in a thin layer of the adsorbent was carried out on L 5/40 M plates in the following solvent systems: tholuene-acetone-25% ammonium hydroxide (50:50:1); ethyl acetate-methanol-25% ammonium hydroxide (17:2:1); chloroform-methanol (9:1); and chloroform-acetone (9:1). Analysis by high-pressure liquid chromatography was carried out by means of a Milichrome-4 chromatographer with a UV detector on the column packed with Separone C18 (5 mcm) using a mobile phase acetonitrile-0.05 M disubstituted ammonium phosphate (55:45).