Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering

Nie1, Emory

  • 1Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.

Science (New York, N.Y.)
|February 21, 1997
PubMed
Summary

Surface-enhanced Raman scattering enables room-temperature optical detection of single molecules and nanoparticles. This technique provides significantly larger Raman enhancement factors than previously achieved, leading to more intense and stable signals than fluorescence.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electron impact fragmentation mechanisms of some cyclic esters with helical structures

Rapid communications in mass spectrometry : RCM·2000
Same author

Study of the Adsorption of Glutathione on a Gold Electrode by Using Electrochemical Quartz Crystal Impedance, Electrochemical Impedance Spectroscopy, and Cyclic Voltammetry.

Journal of colloid and interface science·2000
Same author

Ordered Hierarchical Porous Materials: Towards Tunable Size- and Shape-Selective Microcavities in Nanoporous Channels The Pacific Northwest National Laboratory is operated by Battelle for the U.S. Department of Energy under Contract DE-AC06-76RL0 1830. This work is supported by the Office of Basic Energy Sciences, Division of Materials Sciences, of the U.S. Department of Energy.

Angewandte Chemie (International ed. in English)·2000
Same author

alpha-Amylase immobilized on bulk acoustic-wave sensor by UV-curing coating.

Biochemical engineering journal·2000
Same author

1,2:1,3-bis(mu-p-toluato-O:O')-1-(triphenylphosphine-P)-1-ruthena- closo-undecaborane

Acta crystallographica. Section C, Crystal structure communications·2000
Same author

Real-space imaging of two-dimensional antiferromagnetism on the atomic scale

Science (New York, N.Y.)·2000

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Nanotechnology

Background:

  • Single-molecule detection and spectroscopy are crucial for understanding chemical and biological processes at the molecular level.
  • Conventional spectroscopic methods often lack the sensitivity required for analyzing individual molecules or nanoparticles.
  • Surface-enhanced Raman scattering (SERS) offers a potential pathway to enhance weak spectroscopic signals.

Purpose of the Study:

  • To achieve optical detection and spectroscopy of single molecules and single nanoparticles at room temperature.
  • To utilize surface-enhanced Raman scattering (SERS) for amplifying spectroscopic signatures.
  • To investigate the size-dependent properties of individual silver colloidal nanoparticles for enhanced signal amplification.

Main Methods:

Related Experiment Videos

  • Employed surface-enhanced Raman scattering (SERS) for optical detection and spectroscopy.
  • Screened individual silver colloidal nanoparticles from a heterogeneous population based on size-dependent properties.
  • Utilized selected nanoparticles to amplify the Raman spectroscopic signatures of adsorbed single molecules (rhodamine 6G).

Main Results:

  • Achieved room-temperature optical detection and spectroscopy of single molecules and nanoparticles.
  • Determined intrinsic Raman enhancement factors for single rhodamine 6G molecules on selected nanoparticles to be 10^14–10^15.
  • Observed that these enhancement factors are significantly larger than ensemble-averaged values from conventional measurements.

Conclusions:

  • Demonstrated the capability of SERS for highly sensitive single-molecule and single-nanoparticle spectroscopy at room temperature.
  • The enormous Raman enhancement achieved leads to vibrational signals that are more intense and stable than single-molecule fluorescence.
  • Highlights the potential of SERS using tailored nanoparticles for advanced spectroscopic applications.