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Published on: August 16, 2018
Modulation of a competitive N-methyl-D-aspartate receptor antagonist binding by zinc oxide
1College of Pharmacy, Xavier University of Louisiana, New Orleans 70125, USA.
Abstract:
Zinc through a zinc binding site is known to modulate the binding of agonists at the NMDA receptor. In the present study, the ability of zinc oxide to alter the specific binding of [3H]CGP-39653, a competitive NMDA receptor antagonist, was determined in homogenate of rat brain tissue. Analysis of saturation experiments indicated that zinc oxide significantly increased the Kd without changing Bmax of [3H]CGP-39653 binding. Furthermore, the effect of ZnO on glutamate and glycine displacement of [3H]CGP-39653 binding was determined. The results of the [3H]CGP-39653 displacement study indicated that ZnO decreases the glutamate and glycine displacement of [3H]CGP-39653 binding.
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