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Evaluation of reference values for erythrocyte glutathione
T Cevat Inal1, A Tuli, G T Yüregir
1Cukurova University, Medical Faculty, Department of Biochemistry, Balcali, Adana, Turkey.
This study established reference values for erythrocyte glutathione, finding whole blood stored at -70°C for up to 10 days optimal. These glutathione levels are suitable for diagnostic use.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Hematology
Background:
- Erythrocyte glutathione levels are crucial biomarkers.
- Establishing reliable reference values and storage conditions is essential for accurate clinical diagnostics.
Purpose of the Study:
- To determine analytical, intra-individual, and inter-individual variations of erythrocyte glutathione.
- To identify optimal storage conditions for erythrocyte glutathione.
- To establish reference values for erythrocyte glutathione in a Turkish population.
Main Methods:
- Analyzed erythrocyte glutathione in 396 healthy individuals (206 males, 190 females) from southern Turkey.
- Assessed variations (analytical, intra-individual, inter-individual) in 20 subjects.
- Evaluated different storage conditions for whole blood samples.
Main Results:
- The mean erythrocyte glutathione level was 6.9 (1.0) µmol/gHb, with no significant sex difference.
- Analytical, intra-individual, and inter-individual variations were 4.63%, 13.67%, and 11.16%, respectively.
- Optimal storage was whole blood at -70°C for up to 10 days.
Conclusions:
- Established population-specific reference values for erythrocyte glutathione.
- Identified optimal sample storage for reliable glutathione measurements.
- Index of individuality suggests glutathione reference values are suitable for diagnostic applications.
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