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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein
1Department of Biochemistry, Chinese University of Hong Kong, Shatin, Hong Kong.
Journal of Cellular Biochemistry
|February 1, 1997
Summary
Researchers isolated the full-length human 56 kDa selenium binding protein (hSP56) cDNA, the human homolog of mouse SP56. The gene is located on chromosome 1, and hSP56 was successfully expressed in E. coli.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Selenium binding proteins play crucial roles in cellular processes.
- Understanding human selenium binding protein (hSP56) is important for its biological function.
- The mouse homolog of hSP56 has been previously identified.
Purpose of the Study:
- To isolate and characterize the full-length human 56 kDa selenium binding protein (hSP56) cDNA.
- To determine the gene's chromosomal location.
- To investigate the expression pattern of hSP56 in mouse tissues and its expression in E. coli.
Main Methods:
- Isolation of the full-length hSP56 cDNA clone.
- Northern blot hybridization for gene expression analysis.
- Bacterial expression of hSP56 in Escherichia coli.
Main Results:
- The hSP56 cDNA is 1,668 bp long, encoding 472 amino acids with a calculated molecular weight of 52.25 kDa.
- hSP56 is expressed in mouse heart at an intermediate level compared to other tissues.
- Successful expression of functional hSP56 in E. coli was achieved.
Conclusions:
- The full-length hSP56 cDNA has been successfully isolated and characterized.
- The hSP56 gene is mapped to human chromosome 1q21-22.
- hSP56 exhibits tissue-specific expression and can be recombinantly expressed.
