Cardiotoxicity of verrucotoxin, a protein isolated from the venom of Synanceia verrucosa

Freshly purified but unstable verrucotoxin (VTX) and a more stable proteic complex of the toxin (p-VTX) were isolated from the venom of the stonefish Synanceia verrucosa and applied to frog atrial fibres. VTX and p-VTX decreased the amplitude and the duration of the stimulated peak tension and accelerated the relaxation phase of the contraction. The negative inotropic effect of p-VTX decreased with increasing the external Ca concentration ([Ca]o) in the Ringer solution. The negative chronotropic effect induced by p-VTX was insensitive to change in [Ca]o. It is reversed by glibenclamide. p-VTX shortened the duration of the plateau and the repolarizing phase of the action potential. Glibenclamide but not tetraethylammonium reversed the p-VTX-induced shortening of the AP repolarizing phase. The data suggest that the toxin isolated from the venom of S. verrucosa inhibits Ca channels and might activate ATP-sensitive potassium channels in frog atrial heart muscle.