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Multichannel microchip electrospray mass spectrometry

Q Xue1, F Foret, Y M Dunayevskiy

  • 1Barnett Institute, Northeastern University, Boston, Massachusetts 02115, USA.

Analytical Chemistry
|February 1, 1997
PubMed
Summary
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This study presents a microchip device for electrospray ionization mass spectrometry (ESI-MS). The novel microchip enables sensitive analysis of peptides and proteins, improving ESI-MS applications.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Microfluidics

Background:

  • Electrospray ionization mass spectrometry (ESI-MS) is a powerful analytical technique.
  • Microfluidic devices offer advantages in sample handling and analysis.
  • Integrating microfluidics with ESI-MS can enhance sensitivity and throughput.

Purpose of the Study:

  • To develop and demonstrate a microfabricated multiple-channel glass chip for ESI-MS.
  • To investigate the performance of the microchip for analyzing peptides and proteins.
  • To establish optimal conditions for stable electrospray and sensitive detection.

Main Methods:

  • Fabrication of a multiple-channel glass microchip using photolithography, wet etching, and thermal bonding.
  • Interfacing the microchip with an electrospray ionization mass spectrometer.

Related Experiment Videos

  • Applying individual high voltages to buffer reservoirs for sequential sample spraying.
  • Optimizing liquid flow rate (100-200 nL/min) for stable electrospray.

    • Main Results:

    • Successful interfacing of the microchip with ESI-MS.
    • Achieved a detection limit lower than 6 x 10(-8) M for myoglobin.
    • Demonstrated successful analysis of both aqueous and 75% methanol samples with good sensitivity.
    • Analyzed different standard peptides and proteins in one chip using the parallel multiple-channel system.

    Conclusions:

    • Microfabricated multiple-channel glass chips are effectively interfaced with ESI-MS.
    • The developed system allows sensitive and efficient analysis of peptides and proteins.
    • This technology holds promise for high-throughput proteomic and metabolomic analyses.