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Cloning, functional expression and tissue distribution of rabbit alpha 1d-adrenoceptor
F Suzuki1, S Miyamoto, M Takita
1Department of Pharmacology, Fukui Medical School, Japan.
Biochimica Et Biophysica Acta
|January 14, 1997
Summary
Researchers cloned the rabbit alpha 1d-adrenoceptor, finding its protein structure and function are similar to other mammals. This adrenoceptor is widely distributed in various rabbit tissues, particularly the vas deferens and aorta.
Area of Science:
- Pharmacology
- Molecular Biology
- Genetics
Background:
- Adrenoceptors play crucial roles in physiological processes.
- Alpha 1d-adrenoceptors are important targets for drug development.
- Understanding species-specific adrenoceptor characteristics is vital.
Purpose of the Study:
- To clone and characterize the rabbit alpha 1d-adrenoceptor.
- To investigate its sequence homology and pharmacological properties.
- To determine the tissue distribution of its mRNA in rabbits.
Main Methods:
- Cloning of rabbit alpha 1d-adrenoceptor cDNA from a liver library.
- Expression in COS-7 cells for pharmacological studies.
- Radioligand binding assays.
- Competitive RT/PCR for mRNA distribution analysis.
Main Results:
- A cDNA encoding a 576-amino acid rabbit alpha 1d-adrenoceptor was successfully cloned.
- The deduced amino acid sequence showed high homology to human, rat, and mouse orthologs.
- Expressed receptor exhibited pharmacological binding properties similar to rat alpha 1d-adrenoceptors.
- Alpha 1d-adrenoceptor mRNA was detected in multiple rabbit tissues, with high abundance in vas deferens, aorta, prostate, and cerebral cortex.
Conclusions:
- The rabbit alpha 1d-adrenoceptor shares significant structural and functional similarities with its counterparts in other species.
- The widespread tissue distribution suggests diverse physiological roles for this receptor in rabbits.
- This study provides a foundation for further research into rabbit adrenoceptor pharmacology and function.