Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative decrease of human cytochrome c oxidase during development: evidences for a post-transcriptional

E Lefai1, A Vincent, O Boespflug-Tanguy

  • 1URA CNRS 1940 Université B. Pascal-Clermont II, Aubiere, France.

Biochimica Et Biophysica Acta
|January 16, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuum constitutive laws to describe acoustic attenuation in glasses.

Physical review. E·2020
Same author

Enhancement and anticipation of the Ioffe-Regel crossover in amorphous/nanocrystalline composites.

Nanoscale·2019
Same author

Promoting psychiatrist-pediatrician collaborations on postnatal depression prevention.

L'Encephale·2019
Same author

Propagative and diffusive regimes of acoustic damping in bulk amorphous material.

Physical review. E·2018
Same author

New Simplified Screening Method for Postprandial Hypotension in Older People.

The Journal of frailty & aging·2018
Same author

Mapping between atomistic simulations and Eshelby inclusions in the shear deformation of an amorphous silicon model.

Physical review. E·2016

Cytochrome c oxidase activity declines with age due to reduced protein levels, not changes in gene expression or mitochondrial DNA. This suggests post-transcriptional regulation impacts complex IV function in aging muscle.

Area of Science:

  • Mitochondrial biology
  • Aging research
  • Biochemistry

Background:

  • Cytochrome c oxidase (complex IV) activity decreases with age in human muscle.
  • Complexes I and III activity remain stable during aging.
  • The underlying mechanisms for complex IV decline are not fully understood.

Purpose of the Study:

  • Investigate the causes of age-related decline in cytochrome c oxidase activity.
  • Determine if changes in enzyme kinetics, gene expression, or protein levels contribute to reduced activity.
  • Elucidate the regulatory mechanisms affecting complex IV function during human development.

Main Methods:

  • Measurement of cytochrome c oxidase activity in isolated muscle mitochondria.
  • Analysis of enzyme kinetics (Vmax, Km).

Related Experiment Videos

  • Quantification of cellular mitochondrial DNA (mtDNA) concentration.
  • Assessment of mitochondrial and nuclear gene transcript levels via RT-PCR.
  • Measurement of heme aa3 levels and protein subunit concentrations using antibodies.

    • Main Results:

    • Apparent Vmax of cytochrome c oxidase decreased with age, but Km and mtDNA concentration remained constant.
    • Mitochondrial and nuclear gene transcripts for complex IV subunits increased with age.
    • Heme aa3 levels and protein subunit concentrations (mitochondrial CO II, nuclear CO IV, CO VIIaH) decreased in parallel with enzymatic activity.
    • Complex IV activity reduction is quantitative and linked to post-transcriptional/post-translational events.

    Conclusions:

    • The age-related decrease in muscle cytochrome c oxidase activity is quantitative.
    • Regulation occurs at the post-transcriptional and/or post-translational level, affecting protein abundance.
    • Despite increased gene expression, lower protein levels lead to reduced enzyme function in aging muscle.