Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Salmonella enteritidis outbreak in Thailand: study by random amplified polymorphic DNA (RAPD) analysis

L Kantama1, P Jayanetra

  • 1Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

The Southeast Asian Journal of Tropical Medicine and Public Health
|March 1, 1996
PubMed
Summary

A 1990 Salmonella enteritidis outbreak in Thailand was primarily caused by a single clone. Random Amplified Polymorphic DNA (RAPD) analysis identified this dominant clone, likely originating from chickens, responsible for most human and chicken infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biogeographic distribution of polyploidy and B chromosomes in the apomictic Boechera holboellii complex.

Cytogenetic and genome research·2005
Same author

Is the aneuploid chromosome in an apomictic Boechera holboellii a genuine B chromosome?

Cytogenetic and genome research·2004
Same author

Clinical and prognostic categorization of extraintestinal nontyphoidal Salmonella infections in infants and children.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·1999
Same author

Primers for Salmonella serovar detection by polymerase chain reaction.

The Southeast Asian journal of tropical medicine and public health·1998
Same author

Construction of specific DNA probe for the detection of Salmonella in food.

The Southeast Asian journal of tropical medicine and public health·1997
Same author

Electron microscopy study of the mode of growth of Pseudomonas pseudomallei in vitro and in vivo.

The Journal of tropical medicine and hygiene·1995

Area of Science:

  • Microbiology
  • Epidemiology
  • Molecular Biology

Background:

  • A significant outbreak of Salmonella enteritidis occurred in Thailand starting in 1990.
  • Salmonella enteritidis isolates were predominantly found in chicken and human sources nationwide.
  • The sustained high rate of spread necessitated an investigation into potential clonal origins.

Purpose of the Study:

  • To investigate clonal involvement in the 1990 Salmonella enteritidis outbreak in Thailand.
  • To identify the specific clones responsible for the ongoing epidemic.
  • To determine the potential source and invasive characteristics of Salmonella enteritidis during the outbreak.

Main Methods:

  • Clonal identification of 125 Salmonella enteritidis isolates from 1990-1993.

Related Experiment Videos

  • Utilized Random Amplified Polymorphic DNA (RAPD) analysis for genotyping.
  • Analysis of isolate distribution across different sources (chicken, human, blood).

    • Main Results:

    • Eight distinct clones (profiles no. 1-8) were identified using RAPD analysis.
    • A single predominant clone, profile no. 4, accounted for 93.6% of all isolates.
    • This dominant clone was prevalent in both chicken and human isolates, suggesting chickens as the primary source.
    • Nearly 40% of Salmonella enteritidis isolates were from blood cultures, indicating potential invasiveness.

    Conclusions:

    • The Salmonella enteritidis outbreak in Thailand was largely driven by a single clone (RAPD profile no. 4).
    • Chickens are strongly implicated as the source of this dominant Salmonella enteritidis clone.
    • The high proportion of blood isolates suggests significant invasiveness of Salmonella enteritidis in the Thai population during the epidemic.