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A monolayer coagglutination microplate technique for typing red blood cells
F Llopis1, F Carbonell-Uberos, M D Planelles
1Regional Blood Transfusion Centre, Valencia, Spain.
Background And Objectives:
Serologic agglutination tests have the disadvantage of lack of an objective endpoint that can be easily read and quantitated. We have developed a new method for ABO and Rh typing based on producing a red blood cell (RBC) monolayer on microplates and the mixed hemagglutination reaction.
Materials And Methods:
We have employed a new red cell fixation buffer to prepare the RBC monolayer. As the technique for grouping, we used a mixed agglutination reaction between the RBC monolayer and the RBCs to be typed.
Results:
Compared with an automated hemagglutination system in 34,519 samples, the method is shown to be more sensitive, free of false positive reactions, and cost-effective.
Conclusions:
The microplate coagglutination method is accurate and lower in cost than conventional methods.
