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Related Experiment Videos

Rh haplotypes that make e but not hrB usually make VS

M E Reid1, J R Storry, P D Issitt

  • 1New York Blood Center, N.Y. 10021, USA.

Vox Sanguinis
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

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The Rh phenotype hrB- is often associated with the VS+ phenotype, suggesting a link between aberrant e antigen expression and hrB antigen nonexpression. Further research is needed to understand the diverse molecular bases of hrB-phenotypes.

Area of Science:

  • Blood group serology
  • Molecular genetics of red blood cell antigens

Background:

  • The Rh phenotypes hrB- and VS+ are rare in White populations but more prevalent in Black populations.
  • The high-incidence antigen hrB is typically found on e+ red blood cells.
  • VS+ red blood cells exhibit aberrant e antigen expression (eS).

Purpose of the Study:

  • To investigate the association between hrB- and VS+ phenotypes.
  • To explore the serological and molecular underpinnings of these rare Rh phenotypes.

Main Methods:

  • Conventional serologic methods were employed.
  • A monoclonal anti-hrB-like antibody was utilized.
  • Sixty-five e+ red blood cell samples, appearing hrB-, were analyzed.

Main Results:

Related Experiment Videos

  • Of the 65 samples studied, 59 (91%) were identified as VS+.
  • In VS+ individuals, a leucine-to-valine substitution at amino acid 245 of the RHCE polypeptide is observed.
  • This molecular change is hypothesized to affect alanine 226, leading to the eS phenotype.

Conclusions:

  • The transition from e to eS may cause the nonexpression or reduced expression of hrB, potentially an epitope of e.
  • The leucine-to-valine change at residue 245 does not fully explain all hrB- phenotypes.
  • Diverse molecular mechanisms likely underlie the various hrB-phenotypes, as evidenced by differences between hrB-VS+ and hrB-VS- samples, and variations in C antigen expression within hrB-VS+ samples.