Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neutron scattering studies on chromatin higher-order structure

V Graziano1, S E Gerchman, D K Schneider

  • 1Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.

Basic Life Sciences
|January 1, 1996
PubMed
Summary

Chromatin condensation forms a stable 30 nm filament structure. Deuterated histone H1/H5 is located internally, crucial for higher-order chromatin structure formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation".

Journal of oncology·2020
Same author

Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation.

Journal of oncology·2019
Same author

Application of a chemiluminescence immunoassay system and GC/MS for toxicological investigations on skeletonized human remains.

Forensic science international·2019
Same author

Physical Activity Among Organ Recipients: Data Collected From the Latin American Transplant Games.

Transplantation proceedings·2017
Same author

Effect of cellulose structure and morphology on the properties of poly(butylene succinate-co-butylene adipate) biocomposites.

Carbohydrate polymers·2015
Same author

Structural basis for stop codon recognition in eukaryotes.

Nature·2015

Area of Science:

  • Molecular biology
  • Biophysics
  • Structural biology

Background:

  • Chromatin, the complex of DNA and proteins, undergoes condensation to form higher-order structures.
  • The 30 nm filament is a key intermediate in chromatin packaging, but its precise structure and the role of linker histones remain areas of investigation.

Purpose of the Study:

  • To elucidate the structure and condensation of chromatin into the 30 nm filament.
  • To determine the location of linker histone H1/H5 within the 30 nm filament.
  • To understand the stoichiometry of histone H1/H5 required for stable higher-order chromatin structure.

Main Methods:

  • Small-angle neutron scattering (SANS) was employed to study chromatin structure and condensation.
  • Deuterated histone H1/H5 was used to pinpoint its location within the 30 nm filament.

Main Results:

  • Chromatin condensation increases with ionic strength, reaching a plateau structure with a mass per unit length of 6-7 nucleosomes/11 nm.
  • Linker histone H1/H5 is localized to the interior of the 30 nm filament, consistent with binding to the inner nucleosome face.
  • Stable higher-order chromatin structures appear to require H1/H5 binding to 5-7 contiguous nucleosomes.

Conclusions:

  • The 30 nm chromatin filament is a condensed structure regulated by ionic strength.
  • Linker histone H1/H5 plays a critical role in stabilizing the 30 nm filament by binding to its interior.
  • A specific stoichiometry of histone H1/H5 binding is necessary for the formation of stable higher-order chromatin structures.

Related Experiment Videos