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Myoglobin solvent structure at different temperatures

B V Daniels1, B P Schoenborn, Z R Korszun

  • 1Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.

Basic Life Sciences
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

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Neutron diffraction reveals that water molecules around myoglobin crystals form distinct hydration shells, not disordered solvent. These ordered shells expand with increasing temperature, indicating a phase transition around 180K.

Area of Science:

  • Biophysics
  • Structural Biology
  • Crystallography

Background:

  • Understanding protein hydration is crucial for biological function.
  • Previous studies suggested disordered solvent around protein crystals.
  • Myoglobin serves as a model protein for studying hydration dynamics.

Purpose of the Study:

  • To investigate the structural organization of water molecules surrounding myoglobin crystals.
  • To determine if the solvent exhibits ordered hydration shells or disordered structure.
  • To analyze the effect of temperature on protein hydration structure.

Main Methods:

  • Neutron diffraction data collection at multiple temperatures (80K, 130K, 180K, 240K).
  • Analysis of low-resolution data using Relative Wilson Statistics.

Related Experiment Videos

  • Quantification of solvent structure using the liquidity factor (Bsn) as a function of distance from the protein surface.

    • Main Results:

    • Evidence of a phase transition in the solvent structure around 180K.
    • Identification of two distinct, well-defined hydration shells surrounding the myoglobin crystals.
    • Observation that both hydration shells expand with increasing temperature.
    • The liquidity factor (Bsn) shows minima at approximately 2.35Å and 3.85Å from the protein surface.

    Conclusions:

    • The solvent structure around myoglobin crystals is not disordered but organized into specific hydration shells.
    • Temperature significantly influences the expansion and structure of these hydration shells.
    • A phase transition occurs around 180K, affecting the solvent's structural organization.