Related Experiment Video
Updated: Aug 4, 2026

Ratiometric Imaging of Extracellular pH in Dental Biofilms
Published on: March 9, 2016
The pH value changes in the periodontal pockets
T Watanabe1, W Soeda, K Kobayashi
1Department of Geriatric Dentistry, Faculty of Dentistry, Tokyo Medical and Dental University.
Abstract:
Many studies have been done of the pH values in periodontal pockets. These studies have suggested that the pH value was influenced by the condition of the surrounding tissue. Thus, the measurement of pH in the periodontal pockets would be effective data to use for diagnosis in periodontal tissue. The sensors which have been used for measuring pH had many disadvantages, however, such as large size, slow response speed, instability in chemical substances, weak physical strength, thermal drift and so on. Therefore, there have been few reliable data on the pH values in periodontal pockets. We developed a new type of pH sensor, which was designed for use in the periodontal pocket. The sensor was made by D.C. reactive sputtering of iridium oxide on solid substances. This sensor was easy to miniaturize, had quick response speed, and was stable with respect to chemical interfere, physical shock, thermal changes, and low electrical resistance. It was used in the present study to measure the pH values in the periodontal pockets of 16 subjects for 7-73 weeks. Our results showed that the pH values in the periodontal pockets differed among the measuring points and were changeable. The pattern of change was similar in the same subject. The pH value changed depending on tooth condition. The salivary pH showed no correlation with that in the periodontal pockets.
Related Concept Videos
Indicators
pH Regulation in Cells
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
pH Homeostasis
Respiratory Regulation of...
Diagnosing Acidosis and Alkalosis
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2 and HCO3− values are examined to...
Factors Influencing Microbial Growth: pH
The Oral Microbiota

