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Characterisation of atypical biotype 3, serotype O:3 Yersinia and development of a simple identification scheme
J N Pham1, S M Bell, I Guilvout
1Department of Microbiology, Prince of Wales Hospital, Randwick, New South Wales, Australia.
Abstract:
Five clinical strains of Yersinia isolated in Japan and identified as atypical biotype 3 or biotype 3B, serotype O:3, phage type II (3*/O:3/II) Yersinia enterocolitica were characterised since the biochemical reactions of these strains indicate they might also belong to the species Yersinia bercovieri. Biochemical tests, characterisation of the beta-lactamases and DNA-DNA hybridization studies provided strong evidence indicating that these strains should be classified as Yersinia enterocolitica. A simple scheme combining a disc diffusion test and four biochemical tests was devised for identification of these atypical strains.
Insights
Five atypical Yersinia enterocolitica strains were identified using biochemical tests and DNA hybridization. These studies confirmed their classification as Y. enterocolitica, not Y. bercovieri, and led to a simple identification scheme.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Diagnostics
Background:
- Atypical Yersinia enterocolitica strains present diagnostic challenges.
- Biochemical profiles of some Yersinia strains suggest potential misclassification.
- Accurate identification of Yersinia species is crucial for public health.
Purpose of the Study:
- To characterize five atypical Yersinia enterocolitica strains isolated in Japan.
- To determine the correct species classification for these strains, distinguishing between Yersinia enterocolitica and Yersinia bercovieri.
- To develop a simplified identification scheme for these atypical Yersinia strains.
Main Methods:
- Biochemical testing was performed on the isolated Yersinia strains.
- Characterization of beta-lactamase activity was conducted.
- DNA-DNA hybridization studies were employed for genetic relatedness assessment.
- A disc diffusion test was incorporated into the identification scheme.
Main Results:
- Biochemical reactions initially suggested possible classification as Yersinia bercovieri.
- DNA-DNA hybridization and biochemical characterization strongly supported classification as Yersinia enterocolitica.
- The strains were confirmed as atypical biotype 3 or 3B, serotype O:3, phage type II (3*/O:3/II) Yersinia enterocolitica.
- A straightforward identification scheme using a disc diffusion test and four biochemical tests was successfully devised.
Conclusions:
- The characterized strains are definitively Yersinia enterocolitica, despite initial atypical biochemical profiles.
- The developed identification scheme provides an effective method for recognizing these atypical Yersinia enterocolitica strains.
- Accurate identification of Yersinia enterocolitica is essential for appropriate clinical and epidemiological management.