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Improvement of blood and fly gut processing for PCR diagnosis of trypanosomosis
L Penchenier1, V Dumas, P Grebaut
1Laboratoire de recherche sur les trypanosomes OCEAC, BP 288, Yaoundé, Cameroun.
Parasite (Paris, France)
|December 1, 1996
Abstract:
We have adapted a simple and efficient technique to detect trypanosomes in human blood, without DNA purification, and increased the sensitivity threshold to 1 parasite in 1 ml. We have then applied it for detection of parasites in midguts of tsetse flies, negative by microscopy. This technique has been developed for field conditions and could greatly facilitate epidemiological studies.