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Related Experiment Videos

A transgenic marker for mouse B lymphoid precursors

I L Mårtensson1, F Melchers, T H Winkler

  • 1Department of Cellular and Molecular Biology, Immunology Group, Lund, Sweden.

The Journal of Experimental Medicine
|February 17, 1997
PubMed
Summary

Transgenic mice expressing human CD25 revealed a novel precursor cell in B cell development. This finding advances understanding of early B lymphocyte differentiation and immune system development.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Genetics

Background:

  • The lambda5 gene is crucial for precursor (pre)-B cell development.
  • CD25 is a marker involved in immune cell regulation and proliferation.

Purpose of the Study:

  • To investigate the regulatory elements controlling lambda5 gene expression.
  • To identify novel cell populations during B cell development using human CD25 as a reporter.

Main Methods:

  • Generation of transgenic mice expressing human CD25 under the control of the lambda5 5' regulatory region.
  • Flow cytometry analysis of B lineage cells to identify novel cell populations.
  • Analysis of immunoglobulin gene rearrangement status in precursor cells.

Main Results:

  • Transgenic mice expressed human CD25 in parallel with endogenous lambda5 on pre-B cells.
  • A new B220+CD19-lambda5+ precursor cell population was identified.
  • This novel precursor population, along with pre-BI cells, could be cloned on stromal cells with interleukin-7.

Conclusions:

  • The 722-bp region 5' of the lambda5 gene confers tissue and differentiation stage-specific expression.
  • The identified CD19- precursor cells have immunoglobulin heavy chain loci in germline configuration, preceding DJH rearrangement in pre-BI cells.

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