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Related Experiment Videos

Yeast telomeric sequences function as chromosomal anchorage points in vivo

A Mirabella1, M R Gartenberg

  • 1Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.

The EMBO Journal
|February 3, 1997
PubMed
Summary

Yeast telomeric DNA sequences can anchor DNA, preventing its rotation. This DNA anchoring is mediated by Rap1p binding sites and an unknown factor, independent of SIR genes.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Telomeres are protective caps at the ends of eukaryotic chromosomes.
  • Telomeric DNA sequences are known to interact with various proteins.
  • The dynamic nature of DNA, including rotation, is crucial for cellular processes.

Purpose of the Study:

  • To investigate the role of yeast telomeric sequences in DNA topology and rotation.
  • To identify factors involved in telomeric DNA anchoring.
  • To understand the structural basis of telomere function in Saccharomyces cerevisiae.

Main Methods:

  • Site-specific recombination in Saccharomyces cerevisiae to create non-replicative DNA rings.
  • Utilizing topoisomerase mutants expressing Escherichia coli topoisomerase I.

Related Experiment Videos

  • Analyzing DNA topology of telomeric DNA rings using synthetic and mutant sequences.
  • Investigating the role of Rap1p and SIR genes (SIR2, SIR3, SIR4) in DNA anchoring.

    • Main Results:

    • Yeast telomeric DNA sequences, specifically C1-3A/TG1-3 fragments and Rap1p binding sites, create a barrier to DNA axial rotation.
    • DNA anchoring is dependent on an unidentified limiting trans-acting factor and is diminished by competing telomeric sequences.
    • Deletion of Rap1p C-terminal domain and disruption of SIR genes did not affect telomeric DNA ring topology.
    • A SIR-independent protein-DNA assembly linked to an insoluble nuclear structure is proposed to cause DNA anchoring.

    Conclusions:

    • Yeast telomeric DNA sequences possess intrinsic properties that can anchor DNA, hindering its rotation.
    • The anchoring mechanism involves Rap1p binding sites and a trans-acting factor, forming a SIR-independent complex.
    • This DNA anchoring may play a role in telomere maintenance and function within the nucleus.