Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrically induced NGF production by astroglial cells

S Koyama1, T Haruyama, E Kobatake

  • 1Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

Nature Biotechnology
|February 1, 1997
PubMed
Summary

Electrical stimulation of astroglial cells on electrodes induces nerve growth factor (NGF) production. This novel method shows potential for neurotrophic factor research and therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fabrication of boron-containing apatite ceramics via reaction sintering route and their response of lymphocytes for adoptive immunotherapy.

Biomaterials and biosystems·2026
Same author

In vivo responses of β-tricalcium phosphate-based paste-like artificial bone with antibacterial properties under potentially infectious conditions using a rabbit model.

Journal of materials science. Materials in medicine·2025
Same author

Efficacy of avelumab plus axitinib versus sunitinib by numbers of IMDC risk factors and target tumor sites at baseline in advanced renal cell carcinoma: long-term follow-up results from JAVELIN Renal 101.

ESMO open·2023
Same author

Relationship between the combination of polyunsaturated fatty acids intake and psychological distress during pregnancy: The Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study.

Prostaglandins, leukotrienes, and essential fatty acids·2022
Same author

Gastrectomy with or without omentectomy for cT3-4 gastric cancer: a multicentre cohort study.

The British journal of surgery·2020
Same author

Thermo-, photo-, and mechano-responsive liquid crystal networks enable tunable photonic crystals.

Soft matter·2017

Area of Science:

  • Neuroscience
  • Biotechnology
  • Cell Biology

Background:

  • Nerve growth factor (NGF) is crucial for neuronal survival and growth.
  • Astroglial cells are key players in the central nervous system and can produce neurotrophic factors.
  • Controlled induction of NGF production is desirable for research and therapeutic strategies.

Purpose of the Study:

  • To develop a controlled culture system for inducing nerve growth factor (NGF) production in astroglial cells.
  • To investigate the effects of electrical stimulation on NGF production and secretion.
  • To explore the underlying molecular mechanisms, specifically the role of protein kinase C (PKC).

Main Methods:

  • A controlled culture system was established using astroglial cells cultured on an electrode surface.

Related Experiment Videos

  • Electrical stimulation was applied by modulating electrode potential (300 mV amplitude, 10 Hz frequency).
  • NGF production and secretion were measured, and the involvement of protein kinase C (PKC) was assessed.

    • Main Results:

    • Electrical stimulation successfully induced NGF production and secretion in astroglial cells.
    • The applied electrical parameters (300 mV, 10 Hz) were effective in triggering this response.
    • Evidence suggests protein kinase C (PKC) activation is involved in the mechanism of electrically induced NGF production.

    Conclusions:

    • A novel method using electrical stimulation can reliably induce NGF production in astroglial cells.
    • This technique offers a controlled approach for generating NGF, with potential applications in neuroscience research.
    • The findings highlight the role of PKC activation in mediating the cellular response to electrical stimulation for NGF synthesis.