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Updated: Oct 2, 2026

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
Published on: April 27, 2018
[Inhibitors of the ADP/ATP antiporter induce two Ca2+-dependent uncoupling systems in rat liver mitochondria]
L M Mikhaĭlova1, Iu E Kushnareva, A Iu Andreev
1Department of Pharmacology and Experimental Therapeutics, University of Maryland, School of Medicine, Baltimore 21201, USA.
Abstract:
Effect of an ADP/ATP antiporter inhibitor carboxyatractylate on permeability of inner mitochondrial membrane was studied. Carboxyatractylate induced two uncoupling systems: Ca(2+)-dependent pore and another system which was sensitive to an inhibitor of calcium uniporter ruthenium red (apparently, Ca(2+)-recycle). Extent of Ca(2+)-recycle induction correlates with a fraction of mitochondrial population which underwent permeabilization; inhibition of permeabilization by cyclosporin A prevents induction of the recycle. Thus, induction of Ca(2+)-dependent pore is direct consequence of carboxyatractylate binding to mitochondria whereas induction of Ca(2+)-recycle is indirect. Both uncoupling systems are induced by carboxyatractylate concentrations which are specific for ADP/ATP antiporter. The data suggest that the antiporter can directly participate in the formation of Ca(2+)-dependent pore in inner mitochondrial membrane.
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