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Related Experiment Videos

Transfection with aFGF cDNA improves wound healing

L Sun1, L Xu, H Chang

  • 1Resuscitative Medicine Program, Naval Medical Research Institute, Bethesda, Maryland 20889-5607, U.S.A.

The Journal of Investigative Dermatology
|March 1, 1997
PubMed
Summary
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Somatic gene therapy using acidic fibroblast growth factor (aFGF) plasmid significantly accelerated wound closure and improved wound strength in diabetic mice. Gene delivery enhanced healing through sustained aFGF expression, demonstrating therapeutic potential.

Area of Science:

  • Regenerative Medicine
  • Molecular Biology
  • Wound Healing Research

Background:

  • Somatic gene therapy offers a novel approach for delivering therapeutic agents like growth factors to improve wound healing.
  • Diabetic mice (db/db) exhibit impaired wound healing, making them a relevant model for studying therapeutic interventions.

Purpose of the Study:

  • To evaluate the efficacy of acidic fibroblast growth factor (aFGF) gene therapy in accelerating wound healing in a mouse model.
  • To assess the impact of aFGF gene delivery on wound closure, strength, and tissue quality in diabetic mice.

Main Methods:

  • Experimental excisional and incisional wounds were created in diabetic mice (db/db).
  • Treatment involved topical application of recombinant aFGF or transfection with a eukaryotic expression plasmid encoding aFGF, using cationic liposomes to facilitate uptake.

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  • Gene expression was confirmed using RT-PCR, immune dot blot, and immunohistochemistry.

    • Main Results:

    • Both recombinant aFGF and aFGF plasmid transfection significantly accelerated excisional wound closure (p < 0.05).
    • Incisional wounds treated with aFGF or plasmid showed significantly increased wound-breaking strength (p < 0.05).
    • Quantitative histology indicated improved wound quality in plasmid-treated incisional wounds, with transient gene expression observed.

    Conclusions:

    • Somatic gene delivery of aFGF effectively promotes wound healing in a diabetic mouse model.
    • aFGF gene therapy leads to both gene expression and functional improvements in wound healing, suggesting its therapeutic potential.