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Crystallization and preliminary x-ray analysis of recombinant staphylokinase
A Rabijns1, K Baeyens, H L De Bondt
1Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
Proteins
|January 1, 1997
Abstract:
Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl2, Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions alpha = 60.6 A, b = 43.7 A, c = 54.3 A, and beta = 115.6 degrees. A complete native data set to 1.8 A resolution has been collected using synchrotron radiation.