Crystallization and preliminary x-ray analysis of recombinant staphylokinase
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Updated: Oct 13, 2026

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
Published on: April 11, 2021
A Rabijns1, K Baeyens, H L De Bondt
1Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
Abstract:
Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl2, Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions alpha = 60.6 A, b = 43.7 A, c = 54.3 A, and beta = 115.6 degrees. A complete native data set to 1.8 A resolution has been collected using synchrotron radiation.
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