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Opioid effects on macrovascular dopamine release

S Scholz1, H Al-Kharrat, B Nibbio

  • 1Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, 19107, USA.

General and Comparative Endocrinology
|February 1, 1997
PubMed
Summary

Opioid substances, including codeine and met-enkephalin, stimulate dopamine release from eel blood vessels. This suggests opioid regulation of dopamine secretion in vascular systems, with unique responses to naloxone.

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Area of Science:

  • Neuroendocrinology
  • Pharmacology
  • Vascular Biology

Background:

  • Alkaloid opiates and met-enkephalin are found in vertebrate chromaffin cells, influencing catecholamine secretion.
  • Dopamine (DA) release from eel and rat large blood vessels originates from unidentified sources.
  • Opioid substances play a role in regulating various physiological processes, including neurotransmitter secretion.

Purpose of the Study:

  • To investigate the impact of alkaloid opiates and met-enkephalin on dopamine secretion from American eel macrovessels.
  • To determine the sensitivity of dopamine release in large blood vessels to opioid substances.
  • To explore potential receptor and effector systems involved in opioid-mediated dopamine release.

Main Methods:

  • Utilized a perifusion system to study dopamine secretion from three macrovessels of the American eel (Anguilla rostrata).

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  • Administered codeine, morphine, and met-enkephalin to assess their effects on DA release.
  • Applied the antagonist naloxone to evaluate its impact on DA secretion.

    • Main Results:

    • Codeine, morphine, and met-enkephalin significantly increased DA release from the ventral aorta and caudal vein.
    • Naloxone stimulated DA release from the caudal vein but had no effect on the ventral aorta.
    • Codeine was the only substance to significantly affect DA release from the posterior cardinal vein.

    Conclusions:

    • Dopamine release from eel macrovessels is sensitive to opioid substances.
    • Antagonistic effects between alkaloid opiates and opioid peptides, observed elsewhere, appear absent in these large blood vessels.
    • The stimulatory effect of naloxone in the caudal vein suggests the involvement of unidentified receptor and/or effector systems.